?�s*�N�c>w��ZQ��\d� ��^Z�L ϸ�u!Qk��%Wd��b2 ^yŪ�zz/9��= �GG�ZGG��� �L� �!D�ut40��� �@ J � ĸLD���@, `�gQޠ���зP�ѫ��.N���o�O\�p��m}�L,�,d�z� #X0�3��SҌ��� ��)i6�#`q� �� .�U�` |6~a %PDF-1.5 %���� u�:u#o|�������T�z���w�������ZB)̯���Ëm��JE(�]��9W�FG7�9ڹ� ��D`ws�-:�D�'� Kc? endstream endobj 808 0 obj <>stream This spectrum was collected by in the summer of 1995 using a Spex FluoroMax. '���w�9���$��V������T�M�-�>|8<>��a1�Mc�6l�f4)�4W�(Vql����{�U�!�q-��W�"�T����L�(�NF^\�2��ZFV����E'/�O����G��K�9�H7{�Z�$���Ċ=.d�E�� {�(t���s$d���LȓQ��=H'�2we��JZQ�A��3�7IM��l�أK�dlǭ�2畝4�d��+l��W��[�qMd��9�� k7�� �{b���9����h�~�X+�K��XᄖR���Hm �8j@V =�T�݂wPOO��h�r�n'�n|(��x�6!wD�7x�x�Y}I����U(��J��`����mxM�ͨ� endstream endobj 804 0 obj <>/Metadata 70 0 R/Outlines 172 0 R/PageLayout/OneColumn/Pages 799 0 R/StructTreeRoot 213 0 R/Type/Catalog>> endobj 805 0 obj <>/Font<>>>/Rotate 0/StructParents 0/Type/Page>> endobj 806 0 obj <>stream |�ࡺ�hO?�͞{�.=��T�V$�5V��j��Թ��. The ratio of the intensities between the third and the first vibronic components of the pyrene emission spectrum (bands I at 373 nm and III at 385 nm) (Fig. Copyright © 2014 Elsevier B.V. All rights reserved. �Cd�>��yR�PEQ�n- 2�W���]�=)^; �8e3�S����$HE�zt5:9C5N�P�a�u����JY�F9���w�ڶݿ-~�(j_�7��CJ����Q:(bG�&� �6MԎ~ ���˃����El3Z�A'*���м.����&��b�4,_�!��s�C���_��9�����c4�s�/�t������S�LH�S��rے���FFFTuS��R'��X�JO�A����X���E��s|'M0^4d��%KsMtC��L��rK�i�w ��x�N��x H��VMo�6��W̑l-Z�HJZ,�'��M��U���A���Grm9������qt�=Հ%~���㛡&�nqUV�?9躲���0�� Copyright © 2020 Elsevier B.V. or its licensors or contributors. ��j�_�|E��o��[ ��}7��x��_�(���b/�/��Mq���'��8j�GN�4�������&*�K��1��wj�$ZW�>�$r�$����W��~���I���=1s�0s?�8�J�-�u���5�����oy����l���zU�W�r�f�f��;y�m�պ��*��3����r8�}�m{|ڭ�r�l���7yY�Ou���]��~�i��� .>����OQe���)7�նZk0�/�w�l��YQߟm�U�^۳UScfo��j�]����,����$^ ���;���� &25���#&��k�N��� ÿ1A7�� ��'6��)�ݷ����'l���t���� -̼ �-����� 1�"f獘�\21\��:o���7�vj*��f��Ɓ ����� �g 8S)U*�(+�_앳0.���Z��\`��K�K�3�:�l���Ա�;P�@t�|Ci�l��b�ws� �`��d��� 3EC�R�n�mL������`y.f�m~\+�qzW��Yњ ��t��CqyS�S7Q�����uW���.�qI$���b��Q��s���� \�~Du��F�Ћ �W�|�w��%��DՈ��� �{!�B����&N"�ŝ ����:����,���Na" ��rC #{,�� h�Ԛmo�6ǿ The fluorescence spectrum of pyrene shows characteristic vibronic bands around 370 400- nm, whose absolute and relative intensities, width and positions depend sensitively on the polarity of the microenvironment[3,26,40-45]. We apply the model to the determination of the cmc from the pyrene fluorescence intensity, especially from the intensity ratio at two vibronic bands in the monomer emission or from the ratio of excimer to monomer emission intensity. H��VI��6�ϯ��*���[N-z���#�c��b�3�߷P��L�b?�o�������hc��ja�ϼ��D�ic�H�v&��a!�x�]%���Lnw�g��*��+�{�KQ#�?�uQ�5�]�!~��l)ת�B�]�m�6ob!O���w���u��4�bGV�9ҫ���ǒw��> |��(��39�VN�oA���b(K�F��NF�l5'D��Il�~���ߋVI)E���Y�+��u���� C�3�rQ��xAho�Qd����_���SU9>�G8���������r��8"*3�NEV]@��z�� #贬��e�"De.�9H�ߖ%I�-���'����:nZ�`�Q�J�ىy8w��vyMؙ�,�Pj�N�@n{֙��Y�e� H�a��;HZ��A;��wTZ��N1o���ˮ��0+*$1k��?.�̍�1��nZ�KS�����F4�`��8TgoB��,T?EjK�`� �PP�f ��U[��s�AM�)���T]�sڍKn]��H��v��!�S�v�0� �m?���a2�F�k��uY�[+�|��5�O��O ���!������J~.Ig�,��ĖT���+o������;:y������ze��Q$�SA��a�"��Uv�0�x�LҬ�L�B��s$�� The fluorescence emission spectrum of Pyrene dissolved in cyclohexane. '�w;�~5v����k�x��-o/�4��o���>�/�.�/�qa�}���������)G#Y"cQ_j�3;P��4��M �+�kؘ�'�o:�����)W���W�7~�}L�K`M�׏Ϻ �F�F����8������7������SM��K諸��}��]c�������"o�0�_I�$�F���ݮ�ӗ;7��e��Fx�^�?�U�a��Z����IՃ���޽�!������:ș���CO��� �v ��D1��W�Q�jz5�� ��F ~��4!��!���"|��ٗ!�����0�ѽ;� �խ��@� �D:��`:z�U�ۑ�� ��6��h�B�+m%���e�+B�oC��[_��S�>U�K/���_m ?��A놛���_=�Ɓ��;��L\�6�� t^�����=W���������e�VU~���y��B��2�Nx���b ��Ư�U�f��,aQ�S8a�8ş-�nx����@�g��G�7 ����n�9��g��EQ�r��mJ���r�.�P��)��RQy��Z��[�꒘qg��lY�@V�yUB��&����%�{)�B�B�E�d����%����/�(��-��5sȏ�e�3����*�#�P���|ļ9a���{���E�(8�G�"Z$�N�rNWH��ɠ���4V��Ǹ���6-lH�;v��c�M�iʭ�M4*�Q�86��l��j�|7p�d�-7X���M����B���l¹75"�1=��Y�)�: .������-u�QS���0?��$�Ӭ!� ��j�r~�;�#�{C� {j��� V�����9�oC�|;\`�VG1�sTa/K[�L��0��J�`l\���{��_��`�p!��Ă8'p6x�sh����X���^�Y�*Z��tp�B,�5��-f%W>+�Z�Z�ڄچ�u5dEW�P�P"����D�'�?���O2�����d�'����_�Ձ��5|����?����ޗ���/^�����?��r��2!2����3�+*Å�:��.�~��EWo��_�ӗuݴ/^ta5]V��2�bX�3�����N�˭�4�ƃ�e��Q��Ӫ��ؖuKج~��u�58�Q Ԥ>+B�(B�2Ir�)X� 8��H5� f5�e��&j��4�Y �D Z��,D�QC�h�A�j��4@P x�! �Vj��#��w��OG�7��Y����hn*~�4>���&y������@�q�{L�2� �:�� ��E�����3: �DCC[3gDl���D=��9]?�3�1�tj� D�L` ��,6>��f=�9 h��4���>2��ϸ��h�…����Ȫ�{�\Hޒ����)��+���w�����t'�)�r��LflL�8\�xv�&D���X�#�ϗ�H]������V*uH����{ڸ�k�sq�_ J^Ÿ �=� ����@������kF�+� ���� 821 0 obj <>/Filter/FlateDecode/ID[]/Index[803 81]/Info 802 0 R/Length 91/Prev 1516617/Root 804 0 R/Size 884/Type/XRef/W[1 2 1]>>stream h�bbd``b`aӁS���_@��8 _�(,���P����C���g���Vb�v%߮�\���P�����du�!0hj��̟��'rm�9��rA�PK"�Z)��Di�!�Y���w�i�u.a�-�bB� �Ң�$Ls�$aFA� �� �9���3���BDI ��KF�R.8��Bw)�m$�N��"�Z��Dpz�!B�/�Bh�%\�gE�����48j������DKԬ1ʁg���QZ.00� � `�YI�p��M��w� )�N���2?gg�zk�R�j���2��'̚����WeZ֕��Ě����_>�����Ç�_>�y�r����x��xV��q���UZ��K��;���2IØJ=��꧎!�P-��y��������:]W�-��ti���ҷ�ԃIr����#��&� �RߙJw<9m{�٘��� We relate the finite width of the transition region below and above the cmc with the observed changes in the pyrene fluorescence in this region. endstream endobj 809 0 obj <>stream endstream endobj startxref f"�O�ɯ �7�`Q�V�UB.jO����n�D��o��k�3a��+F��9 ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Fluorescence emission of pyrene in surfactant solutions. %%EOF The excitation wavelength was 317nm. 103 M− 1 for Triton X-100 (TX100) and SDS micelles, respectively, show that the partition of pyrene between bulk water and micelles cannot be ignored, even at relatively high surfactant concentrations above the cmc. �|�ERHd�fAe�\�*�� �l� � [���a�/{*ic�):,�-Y.iu�B� The excitation and emission monochromators were set at 1 mm, giving a spectral bandwidth of 4.25 nm. "> � B D���@�����`�.뀄#�F�M �G ���� �� �8y$�E��m����ؕ��x��ڟ;v��-�u~Q`��y�S�5� j��u�i/��������?�g�l��zw����T�?���6厼-?��fS�����Ŧ�}4���^��7��_n|;��lW��f�)��ևN��4��j����.